AICA Ribonucleotide AICAR 50mg

AICA Ribonucleotide AICAR 50mg

Moreover, emerging research indicates that AICAR also have potential benefits treating conditions like obesity, diabetes, and metabolic syndrome. AICAR (5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside), also referred to as ZMP, is a peptide and an analogue of AMP. It is a substance produced naturally by the body that stimulates AMP-dependent kinase activity, a protein that regulates metabolism.

  • AMPK has been found to attenuate inflammatory responses in metabolic disorders in both healthy and diabetic mice.
  • Most importantly, Nrf2 gene deletion markedly weakened the protective effects of AICAR to prevent SAP-induced oxidative stress and NLRP3 inflammasome activation in the liver tissues of L-arginine-induced PALI mice (Figure 7F, Figures 8B,C).
  • Therefore, it is possible that a decrease in SREBP-1c might lead to a reduction in fatty acid synthesis.
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Our AICA Ribonucleotide (AICAR) 50mg has the highest standard of quality with a purity level of 99% or higher. Our peptides for sale are made in the USA, ship within 24 hours of purchase and we offer guaranteed delivery. Shop for AICA Ribonucleotide (AICAR) 50mg and buy peptides online with confidence at Peptide Pros. Immunoprecipitated DNA was recovered using PCR purification kit (Qiagen) and analysed using quantitative PCR. Primers corresponding to human IFNβ promoter (5′-GGGAGAAGTGAAAGTGGGAAA-3′, 5′-CAGGAGAGCAATTTGGAGGA-3′) or IκB (5′-GACGACCCCAATTCAAATCG-3′, 5′-TCAGGCTCGGGGAATTTCC-3′) and IL-8 promoters (5′-GTTGTAGTATGCCCCTAAGAG-3′, 5′-GCCTTTGCATATATCAGACAG-3′) were used for analysis. Data are shown as percentage of input DNA precipitated by a corresponding antibody.

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Our data show that, in addition to NFκB, binding of STAT3 to its response element was also attenuated by AICAR. This correlated with inhibition of STAT3-dependent gene expression by AICAR. In contrast, neither HIF DNA binding, nor HIF-dependent transcriptional activation were inhibited by AICAR in macrophages. Collectively, our results indicate that the ability of AICAR to disrupt an interaction of a transcription factor with its DNA response element may account for the effect of AICAR on transcriptional activation. Structural determinants how AICAR interferes with DNA binding should be revealed in further experiments. High throughput DNA-binding assays may also identify the whole spectrum of transcription factor – DNA interactions sensitive to AICAR.

Nrf2 Knockout Weakens the Protective Effects of AICAR on PALI in L-Arginine-Induced SAP Mice

Although AICAR did not interfere with activation of cytosolic signalling cascades and nuclear translocation of nuclear factor – κB (NFκB) by LPS, it prevented the recruitment of NFκB and RNA polymerase II to target gene promoters. AICAR also inhibited signal transducer and activator of transcription 3 (STAT3)-dependent induction of interleukin (IL) IL-6 and IL-10 targets, while leaving STAT6 and HIF1α-dependent gene expression in IL-4 and dimethyloxalylgylcine-treated macrophages intact. Attenuated gene expression correlated with impaired NFκB and STAT3, but not HIF-binding in electrophoretic mobility shift assays in vitro. Conclusively, AICAR interferes with DNA binding of NFκB and STAT3 to modulate inflammatory responses. Next, we focused on dissecting the deeper molecular mechanism by which AICAR inhibits oxidative stress and inflammation in the liver tissues of sodium taurocholate-induced SAP rats by activating AMPK phosphorylation.

Protein lysates were separated on polyacrylamide gels followed by transfer onto nitrocellulose membranes. Blots were visualized and quantified using the Odyssey imaging system (LICOR Biosciences). Another potential usage for this amazing peptide is after myocardial infarction. After a so-called heart attack, the affected tissue goes through a complex healing process, which we won’t cover in this article. What’s interesting for us, is that the researchers used AICAR to remedy some of the defects in the scar tissue.

AICAR 50mg, also known as 5-Aminoimidazole-4-carboxamide ribonucleotide, is a synthetic molecule that has been shown to activate an enzyme called AMP-activated protein kinase (AMPK) in cells. AMPK plays a crucial role in regulating cellular metabolism and energy homeostasis. By activating AMPK, AICAR can stimulate glucose uptake and fatty acid oxidation in cells, leading to increased energy production and improved insulin sensitivity.